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KMID : 0613820130230091133
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Journal of Life Science
2013 Volume.23 No. 9 p.1133 ~ p.1139
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Specific Detection of Serratia marcescens Based on a PCR Assay and Antimicrobial Susceptibility of S. marcescens Isolated from Boar Semen
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Jung Ji-A
Kim Ae-Ran
Seo Byoung-Joo
Jung Suk-Chan
Kim In-Cheul
Chung Ki-Hwa
Jung Byeong-Yeal
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Abstract
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During the collection of boar semen, bacterial contamination usually occurs. The contamination has deleterious effects both on semen quality and on sow fertility. The majority of contaminants are gram-negative bacteria, especially Serratia marcescens. In this study, we developed a PCR assay for the identification of S. marcescens targeting the luxS gene (GenBank no. EF164926). S. marcescens yielded a specific 306 bp PCR product. However, no amplification was observed in the other strains tested. The detection limit of PCR was 50pg/μl of template DNA of S. marcescens. The antimicrobial susceptibility patterns of S. marcescens isolated from boar semen were tested using the disk diffusion method. Gentamicin, ceftiofur, florfenicol, and neomycin showed high sensitivity in this test. The minimum inhibitory concentration (MIC) was also determined by the broth microdilution method. The MIC_90 values of ceftiofur, enrofloxacin, gentamicin, and neomycin were 8, 8, 8, and 16μg/ml, respectively. These results indicate that PCR amplification of the luxS gene is a reliable and effective method for the identification of S. marcescens and that ceftiofur, enrofloxacin, gentamicin, and neomycin are effective semen extenders for controlling S. marcescens.
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KEYWORD
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Serratia marcescens, boar semen, PCR assay, luxS gene, antimicrobials
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